单细胞 RNA-seq QC

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难度：中等

适用场景：Single-cell teams doing matrix-level QC, annotation, and visualization after count generation. Researchers who need threshold-justified filtering and an auditable record of cells removed or flagged. Teams that want a portable review surface with generated figures, a visualization index, and a notebook or app handoff.

启动提示

Open the visualization index and review notebook or app to inspect QC pass or fail counts, UMAPs, and annotation confidence. Continue in the same thread to refine thresholds, supply a matched reference atlas, or rerun after unblocking doublet detection. Results The run produces a review surface for the filtering decisions, not just a filtered matrix. Begin with the threshold-justification plots and the QC summary so you can see how many cells each filter removed or flagged and whether the ...